Pan-cancer analysis of LncRNA XIST and its potential mechanisms in human cancers

Pan-cancer analysis of LncRNA XIST and its potential mechanisms in human cancers

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Background: X-inactive specific transcript (XIST), it has been found, is abnormal expression in various neoplasms.This work aims to explore its potential molecular mechanisms and prognostic roles in types of malignancies.Methods: This research comprehensively investigated XIST transcription across cancers from Oncomine, TIMER 2.0 and GEPIA2.Correlations of XIST expression with prognosis, miRNAs, interacting protens, immune infiltrates, checkpoint markers, mutations of tumor-associated genes and promoter methylation were also analyzed by public databases.

In addition, 98 BRCA samples were collected to investigate XIST expression and evaluate its clinicopathological value.Results: In public databases, compared to normal tissues, XIST was lower Charbroilers in BRCA, CESC, COAD and so on, but increased in KIRC and PRAD.Databases also showed that XIST was a good indicator of prognosis in BRCA, COAD and so on, but a bad one in KIRC, KIRP and so on.From starBase, we found 29 proteins interacting with XIST, and identified 4 miRNAs which might be sponged by XIST in cancers.Furthermore, XIST was linked with immune infiltration, especially T cell CD4+, and was related to over 20 immune checkpoint markers.

Moreover, several tumor-associated gene mutations and promoter methylation were negatively related to its expression.In addition, IHC showed that XIST in BRCA was obviously Cleaners and Solutions - Instrument Solutions lower in comparison of normal tissues and was negatively related to lymph node invasion and TNM stage.Conclusion: In summary, abnormal expression of XIST influenced prognosis, miRNAs and immune infiltration across cancers, especially BRCA.